streak plate method lab report

Label it as 10-1. The streak plate method is a way to physically separate the microbial population and is done by spreading the inoculate back and forth with an inoculating loop over the solid agar plate.

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Does not gouge the agar.

. INTRODUCTION The streak plate procedure or streaking-out a culture is a standard way of obtaining a pure culture. Open the lid of the labeled new plate and streak for isolation. It is essentially a dilution technique that involves spreading a loopful of culture over the surface of an agar plate.

The spread plate technique was used in lab 5 to obtain isolated colonies. A common method for the isolation of a pure culture from a mixture is by streaking plates. 63D Aseptic Technique Dilution Streaking and Spread Plates.

Lab 2 Aseptic Technique and Streak Plates. Show the loop to flame and then let it cool downThen go to the edge of each line of the first quadrant and extend this to second quadrants sets1 Again sterilize the loop and in the same manner streak the third set of quadrants. The main difference shall be how far the plate is turned during each streak.

For Exercise 1-5 you may be working as a team to be preparing streak plates and will observe the professor perform a pour plate method for isolating colonies. Its also used for detecting possible contamination of cultures. The streak plate method is a rapid qualitative isolation method.

Open the lid of the plate sufficiently 45 degrees to introduce an inoculation loop and only for the time it takes to obtain inoculums. The inoculum is streaked over the agar surface to isolate colonies on at least a portion of the plate. Figure 69 Streak plate method of isolation-overview.

The techniques commonly used for isolation of discrete colonies initially require that the number of organisms in the inoculums be reduced. The decrease of bacteria should show that colonies are sufficiently spread apart to affect the separation of the different types of microbes. Ideal results require that only a small amount of inoculum be used and that the surface of the plate be free of droplets of condensed moisture.

To achieve this I will utilize the quadrant streak. Tape the plate closed and incubates the plate. Streak a portion of.

Usually the Streak plate method is done by streaking the specimen directly onto the Agar media plates but in some cases when the there is the more dense population of the organism in the specimen is suspected that a serial dilution of the specimen is done and then the diluted specimen is streaked onto the solidified agar media plates. Repeat the procedure with all the remaining tubes labeling them until 10-6. To begin the materials were gathered those materials were the plates that were.

The unknown was cultured during the first lab experiment using TSA blood agar MacConkey as the media. Lab report example of unknown bacteria identification. Show the inoculating loop to the flame and keep it.

When turning in the lab make sure you include information relevant for BOTH parts. 15 Invert Plate Streaking Plate 1102018 MDufilho 16 Video. Label it as 10-2.

Mix the contents well by swirling the tube upside down a few times. Rotate the plate counter clockwise 90 degrees and cross the prior streaks to pick up some bacteria and spread them into the next quadrant Repeat in all the four quadrants. From the first tube take 1ml of the sample and transfer it to the second tube.

Streaking is done using a sterile tool such as a cotton swab or commonly an inoculation loop. The first lab period of this experiment an unknown was assigned. Quadrant streaks are the best with isolating pure colonies because you separate microbes from a dense population to a smaller and smaller population until you have a pure.

This is a simple rapid method. This lab report has 2 parts. All of these methods dilute or thin out a heavy population of bacteria across an agar surface.

Plate streak the mixed culture solely in one direction across the entire plate in one long motion. Streak plate the original culture is directly diluted across an agar surface using and inoculating loop. A bacterial suspension taken a loop is spread on the surface of a suitable agar medium in such a.

Repeat the technique for the fourth set of quadrants. Streak Plate Method is done by diluting a comparatively large concentration of bacteria to a smaller concentration. Your report must include the following in 1-2 pages.

The plates were cultured using the streak plate technique. 2 Write your lab report in essay format. Using a sterile pipette add 1ml of sample to the first tube of the set.

Incubation Temperature-controlled chamber Microbe multiplies and produces. THE STREAK-PLATE ISOLATION Student Name Microbiology Lab September 23 2014. Incubate all plates at 35-37C for 36-48 hours.

Then single cells will have been isolated from the biomass. Complete the virtual lab in MH Connect. For the other TSA plate using the above procedure as a guide perform the streak plate using the four-phase method.

The unknown that corresponds to this lab report is unknown 19. Streak Plate Method This method is used most commonly to isolate pure cultures of bacteria A part amount of mixed culture is placed on the rumble of an inoculation. Kitchen Streak Plate and Lab Report Chasatie Wisdom BS203OL11808 Introduction In this experiment I am trying to procure a pure sample of my microbe.

You are responsible for making observations about both the pour plates and streak. The plate was incubated at 37 degrees Celsius until the following lab period.

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